Protein ladder on western blot4/16/2024 Protein Molecular Weight Marker “wide Range” (prestained) is ideal for precise sizing of proteins by SDS-PAGE and for Western blotting. Protein Marker “wide Range” 6,5 kD to 270 kD The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. GangNam-Stain Prestained Protein Ladder is very suitable for protein separation from SDS-PAGE, confirmation of Western blot transfer efficiency, CBB staining. Under suggested conditions, GENE’s Protein Ladder resolves 10 major bands in 4-12% Bis-Tris Gel (MES buffer) and after Western blotting to the nitrocellulose membrane. – 2.5 μl per well for general Western transferring.Ħ2.5 mM Tris-H 3PO 4 (pH 7.5 at 25 ☌), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN 3, 33% glycerol. – 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. The ladder is supplied in the gel loading buffer and is ready to use. The Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer). This Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa.
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